DNA-RNA Amplification RT-PCR
1st-Strand cDNA Synthesis Kit [All-in-One]
- Complete system for 1st-Strand cDNA synthesis
- Active at 42~55℃. Optimum at 50℃
- Superior performance in RT-PCR.
- cDNA synthesis with high processivity and sensitivity
Products
Cat.No. | Product | Size | Price(KRW) |
---|---|---|---|
CDNA25 | HelixCript™ 1st-Strand cDNA Synthesis Kit [All-in-One] | 25 rxns | 150,000 |
CDNA50 | HelixCript™ 1st-Strand cDNA Synthesis Kit [All-in-One] | 50 rxns | 270,000 |
CDNA100 | HelixCript™ 1st-Strand cDNA Synthesis Kit [All-in-One] | 100 rxns | 480,000 |
HelixCript™ 1st-Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from total RNA or poly(A)+-selected RNA. This kit uses HelixCript™ Thermo Reverse Transcriptase, a thermostable and RNaseH negative variant of M-MLV RTase, that is active at a temperature range of 42℃ ~ 55℃. At the escalated temperature, the cDNA synthesis is enhanced partially due to the less internal structural formation of the template RNA and increased polymerization activity of RTase. The included RNase inhibitor in this kit protects the template RNA from degradation in the reaction mix and enhances the yield of cDNA. First strand cDNA synthesized with this kit can be directly used as a template in a downstream assay such as PCR or real-time PCR.